
Why Sample Matrix Choice Matters Choosing the right sample matrix is one of the most critical decisions when designing a flow cytometry assay. Each option offers unique advantages and limitations that can directly impact data quality, logistics, and study outcomes. Flow cytometry requires intact, viable cells. The power of flow…

In episode #23 of The Conversational Flow, Adam and Brian tackle the question: When can flow cytometry be applied to non-blood matrices, and what must be considered? Using sputum analysis as a case study, they outline the challenges of clinical trial sample collection, emphasizing the importance of pre-analytical factors like…

Why Immunogenicity in Drug Development Matters Immunogenicity, why do we keep talking about it? Immunogenicity can, in the simplest of terms, be described as a subject’s ability to generate antibodies specific to the dosed protein therapeutic. Immunogenicity can be either a wanted or unwanted reaction. Wanted immunogenicity is the desired…

If you’ve worked in drug development, you already know that the road from discovery to clinical success is long, expensive, and rarely linear. One of the most pivotal checkpoints in that journey is cytotoxicity testing. Whether you’re developing a small molecule, a biologic, or a cell therapy like CAR‑T, the…

Locked Nucleic Acids (LNAs) are RNA analogs that provide more stable Watson-Crick base-pairing. LNA ribonucleotides can be added into RNA and DNA oligonucleotides to increase the affinity of these oligonucleotides for their complementary sequences. In addition, these LNA present oligonucleotides have extraordinary single-nucleotide discrimination as well as enhanced stability, as…

In episode #91 of The Weekly Bioanalysis, John and Dom return to a two-person format to tackle the “gray areas” of bioanalysis, focusing on drug discovery, biomarkers, tissues, and emerging technologies. They discuss how traditional PK assay validation is highly standardized, but areas like early-stage discovery, biomarker qualification, and tissue…

Screening experiments have become the backbone of modern drug discovery and biological research, enabling the evaluation of thousands of compounds simultaneously. However, screening success hinges on two critical factors: (1) selecting appropriate cellular models and (2) designing robust experimental frameworks. Poor choices can lead to irreproducible results, failed clinical translation,…

In biomarker-driven drug development, selecting the right assay format is critical for generating reliable data that informs scientific and clinical decisions. Whether quantifying proteins, immune cell subsets, or complex molecular signatures, the choice between off-the-shelf and customized assays has direct implications for timelines, costs, and how well the assay performance…

In this episode, the hosts introduce Karla Moncada, who shares her professional journey from Flowcore management to her current role at KCAS Bio, and unveils her new KCAS Bio video series, Karla’s Corner, aimed at fostering year-round conversations within the flow cytometry community. Karla emphasizes the value of personal connections…

Join KCAS Bio at the 18th EBF Open Symposium, taking place in Barcelona, Spain, on November 18-20. This event will bring together scientists from around the globe to discuss a broad array of bioanalytical topics. With the theme “Tuning in to Tomorrow,” The EBF Open Symposium will include strategic presentations…

KCAS Bio will participate in the ACT 46th Annual Meeting, taking place November 16-19 in Phoenix, Arizona. The ACT Meeting gathers the toxicology community for four days of scientific exchange, networking, and professional development. With a strong focus on applied toxicology, the meeting features a robust scientific program, including symposia,…

KCAS Bio will participate in the AMP 2025 Annual Meeting & Expo, taking place November 11-15 in Boston, MA. The AMP 2025 Annual Meeting brings together industry leaders and experts focused on advancing molecular diagnostics and precision medicine. The event covers the latest innovations in biomarker discovery, genomic testing, assay…