Flow Cytometry Services

As a leader in non-GLP and GLP/GCP outsourcing of flow cytometry on a global scale, KCAS Bio offers spectral flow cytometry and conventional flow cytometry, as well as cell sorting services, validated panels, and proteomic analysis service to support non-clinical and clinical studies.

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Global leaders in flow cytometry services

With more than a decade of proven scientific success as a global Contract Research Organization specializing in flow cytometry, we are driven by the mindset that scientific excellence should deliver decision-enabling data on programs that matter the most to you. With an expert team in the US and Europe, and a wide range of offerings, we are well-positioned to support you from pre-clinical discovery studies through clinical-stage studies.

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Why Flow Cytometry

Flow cytometry is applicable across a wide range of research areas, including immunology, oncology, stem cell research, microbiology, and drug development. Whether you are studying cell signaling, immune response, or cell cycle dynamics, flow cytometry can be tailored to your specific research needs. This technology offers the potential for powerful biomarker screening paradigms and multi-parameter cellular profiling enabling interrogation of the immune system’s mechanisms at work. The KCAS Bio team has supported a diverse range of immune system studies and delivered customized and qualified flow cytometry solutions to support the most complex pre-clinical and clinical trials. 



Brian Wile, Ph.D. and Adam Cotty, Ph.D. from the flow cytometry site of KCAS Bio – Philadelphia, share some exciting insights about our labs, scientists, projects and use-cases for flow cytometry.

Harnessing the power of flow cytometry 

Flow cytometry is a technique used to detect and measure the physical and physiological characteristics of a population of cells or particles. Tens of thousands of cells can be quickly examined and analyzed. Uses of flow cytometry can include the determination of cellular characteristics and function, biomarker detection, proliferation, toxicology, and diagnosis of health disorders. 

Whether your assay need is simple or complex, when you partner with us, you’ll work with a reliable and dynamic team who is dedicated to advancing your research goals in a timely way. Together, we can unlock new possibilities, drive innovation, and make meaningful contributions to your field of study.

KCAS Bio services: an in-depth look


One of the most routine and yet powerful applications of flow cytometry (surface and intracellular). Get more insights about immunophenotyping by flow cytometry with an in-depth look at the services we make available to you.

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Cell Sorting

Single or bulk cell sorting - optimized methods and staining panels to ensure successful cell sorts for many different target populations.

Data Analysis

We offer a personalized data analysis package while ensuring the delivery of comprehensive and meaningful reportable summaries that address FDA and compliance standards.

Magnetic bead-based enrichment 

We have established several standard protocols to employ cell sorting or magnetic bead separation for the enrichment of a variety of cell populations for activation &/or expansion in culture.

PBMC isolation and cryopreservation

Our team knows that high-quality PBMC isolation and storage are critical to maintaining cell viability and function.

Receptor Occupancy

Quantify the binding of therapeutic molecules to their target receptors.

How we do it

Bioanalytical lab expertise aligned with your preferred courier delivery hours

We know how precious every whole blood sample is in a clinical trial. That's why we staff our clinical processing team with top level scientists and use our robust training processes to make sure that both our bench scientist and remote analysis teams are ready and waiting for samples Monday-Saturday.

High complexity research delivered with straight forward communication

Our senior scientists have more than 15 years of direct flow cytometry experience that they use to make advanced scientific concepts understandable for technical users and procurement teams alike. They participate on calls throughout your project so that you can have direct access to the experts who understand your assay best.

Your project can scale with us

We regularly support sample analysis for phase 1-3 trials. Our proactive forecasting process means that we maintain sufficient capacity across both instruments and personnel to take on new projects when they come in. We make sure that your project and samples won't go out of stability in a prioritization queue.

Cutting edge facility and equipment

You need the latest research tools to make next level advances with flow cytometry. That's why we've invested heavily in a state-of-the-art facility, cell sorting equipment, spectral flow cytometry capabilities and globally harmonized conventional flow cytometers.

You own every piece of your data

From the first development worksheet to the final raw data file, you own every piece of information we generate. Because of this, we're happy to customize documents and share them directly so that there is transparency at every stage, and we can ensure the data is ready for your bioinformatics workflow as soon as you receive it.

Method Development

We begin by first developing a full understanding of the research application of your assay to ensure that the sample matrix, flow cytometry panels, and gating strategies can be designed and optimized accordingly, and assure adherence to all regulatory guidelines and principles (GLP and GCLP). We then leverage our extensive experience and an in-house antibody titration library to efficiently develop assays to support your unique program.

Method Validation

Once an assay has been developed, the validation stage can begin to ensure the assay is robust and reliable. Assay validation is performed to demonstrate that the method displays the required performance characteristics over the designated range of conditions of the study. Unlike many other platforms, flow cytometry lacks target standards from regulatory agencies, so validation strategies and approaches differ across laboratories. However, since flow cytometry is considered an analytical method, the FDA requires clinical flow cytometric studies to meet specific analytical method validation criteria to confirm that the process is fit for its intended use: specificity, stability requirements, linearity, accuracy, detection limits, range, precision, limits of quantitation, and robustness.

KCAS Bio services: functional assays


Apoptosis assay in flow cytometry measures programmed cell death, assessing changes in cell membrane integrity, DNA content, and identifying apoptotic cells based on distinct fluorescence signals.

Cell proliferation 

Cell proliferation assays are useful to determine cell division and measure the expanding population. The use of flow cytometry allows us to implement cell proliferation assays without radioactivity.

Ex vivo stimulation 

In flow cytometry, ex vivo stimulation involves activating immune cells outside the body before analysis, enabling the study of their functional responses and cytokine production.

Intracellular cytokine analysis (ICS)

ICS is an extremely versatile, high-resolution method that allows researchers to examine the intracellular cytokine production profiles in different cell types, in response to various stimuli.


Unlike lysate-based approaches, phosphoflow assays facilitate the detection and analysis of heterogeneous signaling responses in complex biological matrices, with single-cell resolution.

Single Cell Proteomics (Isoplexis)

As an IsoPlexis Certified Service Provider, we offer comprehensive, multiplexed, functional proteomic and metabolomic analysis with the IsoLight™ Proteomics Hub from IsoPlexis.

Suppression assays 

A suppression assay using flow cytometry evaluates the ability of regulatory cells to inhibit the activation and proliferation of target cells, providing insights into immune system regulation.

Technical considerations

Fluorescence Activated Cell Sorting (FACS)

FACS is a powerful tool that provides unmatched utility in translational and clinical research. Cell sorting requires considerable planning and technical optimization. Working with KCAS Bio’s cell sorting experts will help to ensure your cell sorting yields and purities are achieved. Our BD FACSymphony S6 supports cell sorting with up to 50 parameters of detection and 6-way bulk sorting or single-cell sorting. Consider the many ways sorting can enhance your research projects.

Experts in immunophenotyping

Our scientists have hundreds of collective years of experience operating 18 color instruments, and during that time we've developed an assay to identify almost every cell type imaginable. We've worked with T-cells, B-Cells, Myeloid/NK cells, and more in a variety of matrices from standard whole blood to dissociated tissues to urine.

Develop, validate, and scale receptor occupancy assays in global clinical trials

We've developed and validated dozens of receptor occupancy assays for global clinical trials. We understand many of the risks and challenges associated with quantifying specific antigens at single or multiple processing sites. Our experienced staff is available to make recommendations on optimal panel design and validation to give your trial the best chance at successfully testing out pharmacodynamic hypotheses.

Sensitive assays

Assays like phospho-flow, Treg suppression, and intracellular cytokine quantification can be very sensitive to lab technique. Modern immunology workflows require lengthy and meticulous series of biological operations- this can translate into significant variability or compliance issues that can quickly turn the assay into random number generators rather than essential sources of pharmacodynamic information. Our staff have developed and deployed dozens of workflows to analyze phosphorylation, activation, and suppression of cell types of interest, and our record of conference posters and customer testimonials demonstrate why we get so many repeat requests for this type of assay.

Consistent cell preparation for downstream success

All of our assays require meticulous attention to detail from the time a tube arrives in the lab until the time a result is transferred to our sponsors. We bring that same attention to detail to the sample preparation techniques- PBMC isolation, magnetic enrichment, or even cell sorting- to make sure that any sample we process is ready for downstream analysis.

Deep structural understanding of antibodies to troubleshoot assay design

Our expert staff have developed hybridomas as part of screening programs and have experience with thousands of antibodies in multiple matrices. We leverage all of that experience when we develop panels and translate that into reduced timelines and more initial project success.

Tell us how we can help with your project

We've earned our reputation for delivering reliable, error-free data. We understand the importance of speed, flexibility, and consistency and only make promises we can keep.

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Our people

The Full Potential of Flow Cytometry from KCAS Bio – Leading the Way in 2024 Blogs
Read article The Full Potential of Flow Cytometry from KCAS Bio – Leading the Way in 2024

Flow cytometry is a highly sophisticated laboratory technique. Scientists use this procedure to analyze and quantify certain physical and chemical characteristics of cells or particles. In recent years, the prominence of flow cytometry has grown significantly, and understanding why is pivotal. The recognition of the technique’s importance, especially in the…

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Read article Navigating Flow Cytometry: A Quick Overview to Sample Collection and Processing

Flow cytometry is an extremely valuable tool that has become an indispensable part of modern drug development. This is especially the case in the realm of bioanalytical and biomarker services. However, as is the case with any useful tool, it’s critical to wield flow cytometry skillfully. The accuracy and reliability…

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Read article Podcast (The Conversational Flow) Eps #1: “Why Does Flow Cytometry Need Its Own Podcast?”

Flow cytometry is a technique used to detect and measure physical and physiological characteristics of a population of cells or particles. Tens of thousands of cells can be quickly examined and the data gathered is processed by a computer. Uses of flow cytometry can included determination of Cellular…

Agile, responsive, and easy to work with

We prepare and adapt our services based on a deep understanding of your drug development ambitions and wider business objectives.

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