Spectral flow cytometry and the growing array of commercially available fluorophores have led to the development of increasingly complex immunophenotyping panels.  Generating robust data depends not only on a strong panel design but also on the use of appropriate reference controls and a solid understanding of spectral unmixing principles. …

Georges Köhler and César Milstein: The birth of monoclonal antibodies Since their initial invention almost 50 years ago in 1975, monoclonal antibodies have been widely utilized in the biomedical industry. From cancer treatment to diagnostic assays, over 100 monoclonal antibodies have been approved by the FDA, making them a…

Leonard Herzenberg: The father of modern flow cytometry Leonard Herzenberg was one of the most influential immunologists of his time. Committed to interdisciplinary collaboration and innovation, he would bring together physicists, biologists, and clinicians to tackle some of the most challenging questions in immunology. His pioneering groundwork laid the…

Pioneers of RNA Medicine: The Collaborative Journey of Katalin Karikó and Drew Weissman In the field of modern medicine, Katalin Karikó and Drew Weissman are recognized for their significant contributions to groundbreaking innovation, specifically within the areas of RNA biology and immunology. Their collaborative efforts paved the way for…

Two of the scientific experts from KCAS Bio have been leaders in determining the direction of qPCR and ddPCR technology for the industry. Carrie Vyhlidal, PhD and Jonathan Mercier are both part of the American Association of Pharmaceutical Scientists’ working group for PCR-based methodology, and they have recently…

Flow cytometry is a highly sophisticated laboratory technique. Scientists use this procedure to analyze and quantify certain physical and chemical characteristics of cells or particles. In recent years, the prominence of flow cytometry has grown significantly, and understanding why is pivotal. The recognition of the technique’s importance, especially in the…

Flow cytometry is an extremely valuable tool that has become an indispensable part of modern drug development. This is especially the case in the realm of bioanalytical and biomarker services. However, as is the case with any useful tool, it’s critical to wield flow cytometry skillfully. The accuracy and reliability…

Immunophenotyping has undergone a seismic change in less than two decades as panel sizes have increased in complexity from <10 to >40 colors. Let’s explore how immunophenotyping is transforming the field and how KCAS Bio is at the forefront of this…

Biologics are drugs derived from complex molecules like antibodies. Over the last two decades they have re-emerged as…

In recent years, immunology…

Due to its ability to analyze multiple parameters across different cell types within a sample, flow cytometry provides rich and clinically valuable data sets from even small volumes of blood. However, flow cytometry is a challenging platform to master, and requires significant investment in equipment and technical training.