Podcasts
The main discussion of this 30th episode of “The Conversational Flow” centers on automation in flow cytometry, focusing on a recent paper comparing three major sample preparation systems and their impact on efficiency, standardization, and error reduction. Our hosts, Adam and Brian, explore how automation can significantly reduce hands-on time…
Blogs
At KCAS Bio, we supported a therapeutic monoclonal antibody for advanced solid tumors from non-human primate (NHP) toxicology through first-in-human (FIH) dose escalation into phase 2 expansion. This project combined receptor occupancy assays (ROA) by flow cytometry for cell-surface binding on monocytes, immunophenotyping (IPT) for activation markers, and…
Podcasts
In episode #29 of “The Conversational Flow” podcast, our hosts, Brian and Adam, are joined by guests from Accelerating Cancer Immunotherapy Research (ACIR), a nonprofit dedicated to sharing important advances in cancer immunotherapy with the scientific community and the public. Ute Burkhardt discusses her background in cancer vaccines and neoantigen-driven…
Blogs
Reliable immune profiling across global studies depends on methods that perform consistently, regardless of where they are run. We address this question in the white paper entitled “Cross-Site Validation of a 15-Color Spectral Cytometry Panel for Leukocyte Profiling,” documenting a global collaboration between KCAS Bio and its strategic partner,…
Posters & Papers
Discover in this white paper a global collaboration between KCAS Bio and its strategic partner, Crux Biolabs, to harmonize and validate a standardized 15-color spectral flow cytometry panel for pan-leukocyte profiling across multiple sites. If you have any questions about these services or any others offered by…
Podcasts
In episode 28 of “The Conversational Flow”, our hosts, Adam and Brian, discuss why standardization is essential for credible flow cytometry data at scale. They walk through the biggest sources of variability – reagents and lots, sample handling and shipping time, anticoagulants and processing choices (whole blood vs. PBMCs, fresh…
Blogs
Why Cell Stimulation Matters in Flow Cytometry Many flow cytometry users are happy to start an experiment with a general protocol and a question about their specimen: Will my cells make more cytokines or express more markers after activation? In a healthy person, immune cells like B cells, T cells,…
Podcasts
This pre-holiday episode of “The Conversational Flow” dives into how scientific understanding evolves over time, using immunology as a case study to highlight how often researchers underestimate what they don’t yet know. Adam and Brian reflect on historical quotes from medicine and science that confidently declared fields “complete,” only to…
Blogs
Cell therapy clinical trials present unique challenges when it comes to monitoring both pharmacodynamic (PD) and persistence endpoints. Unlike traditional small molecules or biologics, cell therapies involve living products that interact dynamically with the patient’s immune system. Understanding how these cells persist, expand, and function in vivo is critical for…
Blogs
For flow cytometry methods, reproducibility and scalability are crucial as programs move from discovery to the clinic. When it comes to maintaining consistent flow cytometry data over time, across different collection sites, or across multiple clinical studies, there are important challenges to consider. Backbone flow cytometry panels – well-characterized, optimized,…
Podcasts
In the most recent episode of “The Conversational Flow”, Adam and Brian dive into the flow-based Basophil Activation Test (BAT), explaining how it works, why it matters, and how it has evolved since basophils were first identified. They describe how BAT uses markers to assess basophil activation from a simple…
Podcasts
In episode 25 of “The Conversational Flow” podcast, guest David Leclerc, Director of the Cytometry and Antibody Technology (CAT) Facility at the University of Chicago, joins Brian and Adam to share his professional journey and insights into the evolving world of flow cytometry. Leclerc reflects on his early struggles during…