Podcasts
This pre-holiday episode of “The Conversational Flow” dives into how scientific understanding evolves over time, using immunology as a case study to highlight how often researchers underestimate what they don’t yet know. Adam and Brian reflect on historical quotes from medicine and science that confidently declared fields “complete,” only to…
Blogs
Cell therapy clinical trials present unique challenges when it comes to monitoring both pharmacodynamic (PD) and persistence endpoints. Unlike traditional small molecules or biologics, cell therapies involve living products that interact dynamically with the patient’s immune system. Understanding how these cells persist, expand, and function in vivo is critical for…
Blogs
For flow cytometry methods, reproducibility and scalability are crucial as programs move from discovery to the clinic. When it comes to maintaining consistent flow cytometry data over time, across different collection sites, or across multiple clinical studies, there are important challenges to consider. Backbone flow cytometry panels – well-characterized, optimized,…
Podcasts
In the most recent episode of “The Conversational Flow”, Adam and Brian dive into the flow-based Basophil Activation Test (BAT), explaining how it works, why it matters, and how it has evolved since basophils were first identified. They describe how BAT uses markers to assess basophil activation from a simple…
Podcasts
In episode 25 of “The Conversational Flow” podcast, guest David Leclerc, Director of the Cytometry and Antibody Technology (CAT) Facility at the University of Chicago, joins Brian and Adam to share his professional journey and insights into the evolving world of flow cytometry. Leclerc reflects on his early struggles during…
Blogs
As an emerging and growing application, spectral flow cytometry offers capabilities beyond what is possible with conventional flow. Its unique capabilities make it a powerful tool for scientists aiming to extract more information from each sample and push the boundaries of discovery. Join us in this post as we…
Posters & Papers
Discover in this poster presented by Shalini Gautam, PhD, at ICCS 2025, our work on the “Immunophenotypic Characterization of AML Blast Cells: A Pilot Study for Precision Therapeutic Targeting.“ Gautam_ICCS_2025Download If you have any questions about these services or others offered by KCAS…
Posters & Papers
Discover in this poster presented by Heather Evans, PhD, at ICCS 2025, our work on the “Global Validation of a Pan-Leukocyte Panel with Enumeration for Fresh Whole Blood“. Validation Pan-Leukocyte Panel Whole Blood KCAS BioDownload If you have any questions about these services…
Podcasts
In this episode, Adam and Brian explored recent advances in cell sorting technology, focusing on image-activated approaches. Unlike traditional flow cytometry, which relies on voltage signals, these systems capture and analyze cell images in real time to guide sorting decisions, enabling new insights such as subcellular localization and cell-to-cell signaling.
Blogs
What is immunogenicity? Immunogenicity refers to the ability of a substance, such as a New Biological Entity (NBE) or a vaccine, to provoke an immune response when administered in the body. The recognition of the substance as foreign triggers an immune response, which involves the production of antibodies and the…
Blogs
Why Sample Matrix Choice Matters Choosing the right sample matrix is one of the most critical decisions when designing a flow cytometry assay. Each option offers unique advantages and limitations that can directly impact data quality, logistics, and study outcomes. Flow cytometry requires intact, viable cells. The power of flow…
Podcasts
In episode #23 of The Conversational Flow, Adam and Brian tackle the question: When can flow cytometry be applied to non-blood matrices, and what must be considered? Using sputum analysis as a case study, they outline the challenges of clinical trial sample collection, emphasizing the importance of pre-analytical factors like…