Produced by AAPS
Organized by Sciex
Recorded on December 6, 2023
eChalkTalk available here

Webinar Description:

In this webinar, scientists from KCAS Bio, along with specialists from SCIEX Biopharma & Pharma Solutions discuss

• Developing robust assays for PD biomarkers and their challenges
• Strategies for creating calibration curves, including standard addition and the use of heavy (surrogate) standards
• The complexity of multiplexing in biomarker assays, featuring examples from our work in CNS processes, immune response, and various therapeutic areas
• Innovative approaches to resolving biomarker, isomers, managing wide concentration ranges, and strategies for polar biomarkers that defy standard bioanalytical methods

Speaker information:

John Perkins, PhD, Senior Scientific Advisor, KCAS Bioanalytical and Biomarker Services

John R. Perkins is the Senior Scientific Advisor with a focus on LC/MS technologies at KCAS. Previously, he was at Q2Solutions and legacy companies for over 24 years working in quantitative LC/MS, principally with small molecules. His primary focus was on validation and sample analysis processes as well as managing customer relationships in Ithaca NY. More recently he was responsible for the bioanalytical lab in Oss, The Netherlands. John received his Ph.D. in Supercritical Fluid Chromatography/Mass Spectrometry from University of Wales, College of Cardiff, UK. He did postdoctoral research on nanoscale separation techniques with mass spectrometry at NIEHS in Research Triangle Park. He earned his Bachelors degree in Chemistry from University of Reading, UK and a Masters degree in Advanced Analytical Chemistry from University of Bristol, UK.

Dawn Dufield, PhD, Senior Director, Biopharmaceutical LC-MS/MS Services, KCAS Bioanalytical and Biomarker Services

Dawn R. Dufield is the Senior Director of Biopharmaceuticals (LC-MS/MS) Services at KCAS. Previously she had been with Pfizer and legacy companies for over 20 years working in the quantitative LC-MS/MS field. She was one of the early pioneers of using immunoaffinity combined with LC-MS/MS to offer additional selectivity which is now commonly referred to as Hybrid LCMS. She currently is a member of the AAPS Mass Spec Protein Bioanalysis Committee (MSPBC) and an active member of the American Society for Mass Spectrometry (ASMS). She has numerous publications and presentations in her field and recently contributed to a white paper on recommendations for validation of LCMS based bioanalytical methods for protein biotherapeutics.

Dawn received her Ph.D. in Bioanalytical Chemistry (Honors) from the University of Kansas in Lawrence, K. She earned her bachelor’s degree in Chemistry, (Computer minor) from Southwest Missouri State University in Springfield, MO.

Who Should Attend:

  • Individuals who need to monitor the pharmacodynamics (PD) of their therapeutic drug
  • Individuals who wish to develop and analyze multiplex panels over different concentration ranges
  • Individuals who need to develop and qualify assays for lipid biomarker panels and other challenging small molecule biomarkers

What You Will Learn:

  • Addressed Challenges:
    • Resolution of biomarker isomers within complex panels
    • Maintaining sample throughput during the resolution process
    • Dealing with wide concentration ranges of analytes within specific panels
  • Highlighted Strategies:
    • Addressing resolution issues for biomarker isomers
    • Managing sample throughput in complex panels
    • Developing and analyzing multiplex panels over different concentration ranges
    • Measurement strategy for polar biomarkers not amenable to standard bioanalytical approaches

If you’d like to hear more about Lipid Panels & Small Molecule Biomarkers in Drug Discovery, check out the recording of this eChalkTalk here.