How does immunogenicity testing work?

By systematically narrowing from broad detection to functional relevance, our immunogenicity testing workflow at KCAS Bio provides the reliable data needed to support safety, efficacy, and regulatory expectations.

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Immunogenicity Process

The immunogenicity testing process follows a structured progression, moving from initial ADA detection to full functional and safety characterization:

  • Screening Assay
    All samples are first tested to identify potential ADA-positive results.
  • Negative Reporting
    Samples that do not show ADA activity are reported as negative.
  • Confirmation Assay
    Samples that screen positive move to confirmation to verify true ADA binding.
  • Negative Reporting
    Samples that do not show ADA activity in the confirmatory assay are reported as negative.
  • Titer Assay
    Samples that confirm positive move into a titering assay. When needed, samples are evaluated for neutralizing potential to see whether ADAs interfere with the drug’s function.
  • Neutralizing Antibody (NAb) Assay
    NAb-positive samples are further titrated to assess the strength of neutralization.
  • Tiered Workflow
    The process ensures accurate detection, confirmation, and characterization of the immune response to the therapeutic.
Multi-Tiered Immunogenicity Testing Strategy_KCAS Bio

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