How does flow cytometry work?

Flow Cytometry Process Overview

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Step 1: Sample Preparation (Cellular)
Cells from blood, tissue, or culture are suspended in fluid, filtered if needed, and stained with fluorescent antibodies targeting specific markers. Samples are then washed, possibly fixed, and stored to preserve viability and marker integrity.
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Step 2: Data Aquisition
Prepared samples enter the flow cytometer, where cells pass single-file through a laser beam. Fluorescent signals and light scatter are detected in real time, generating raw data.
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Step 3: Data Analysis
Software converts detector signals into metrics such as size, granularity, fluorescence intensity, and population frequency. Gating strategies define and evaluate specific cell subsets.
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Step 4: Reporting
Results are visualized with plots or tables, then compiled into reports for regulatory, scientific, or internal use.

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