A Masterclass in Advanced Analytical Assays for Bioanalysis of Oligonucleotides

KCAS Bio July 24, 2025

Exploring cutting-edge methods for analyzing oligonucleotides in biological matrices.

Oligonucleotide therapeutics have gained recent popularity given their vast applications in genetic disorders, cancers and infectious diseases. There are many challenges when developing bioanalytical methods for the quantitation of oligonucleotides, including stability, recovery, non-specific binding, chromatographic retention and ionization. In this webinar, case studies with various oligonucleotide structures will be presented along with strategies on how to overcome bioanalytical challenges.

Thursday 31 July 2025
07:00 [PDT] 10:00 [EDT] 15:00 [BST]

Key Topics Explored:

Best practices for oligonucleotide sample preparation
Strategies to ensure the best setup for chromatography and ionization
How to reach good quantitative performance using innovative analytical tools

About the Expert Speakers:

Rathna Veeramachaneni, PhD, Director, Biopharma LC-MS/MS KCAS Bio (KS, USA) Rathna has worked on many different biopharmaceutical modalities such as oligonucleotides (antibody-oligonucleotide conjugates, duplexes and antisense oligonucleotides), monoclonal antibodies, antibody-drug conjugates, fusion and chimeric proteins, as well as biomarkers for both small and large molecules, including glycosphingolipids lipids and eicosanoids. Rathna has significant expertise in hybrid LC–MS/MS, both at the protein and peptide level, along with more traditional LC–MS/MS assays utilizing protein precipitation, digestions and SPE approaches. Her therapeutic area of expertise includes indications of cancer, inflammation, neurodegenerative and rare diseases.