Luminescence Testing

Luminescence Testing

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Luminescence immunoassays use the low-temperature emission of light (as by a chemical or physiological process) and are a variation of the standard ELISA assay. There are many types of luminescence assays including chemiluminescence, electrochemiluminescence, and bioluminescence, which are the three main types that KCAS performs.

Both chemiluminescence and bioluminescence assays use similar enzymes as ELISA, such as horseradish peroxidase (HRP) and alkaline phosphatase (AP). However for luminescence assays, substrates such as luminal, polyphenols, and luciferin are used with HRP while AMPPD (3-(2′-spiroadamantane)-4- methyl-4-(3′-phosphoryloxyphenyl-1, 2- dioxetane, disodium salt) is the substrate most commonly used with AP.  Electrochemiluminescent labels like ruthenium generate light when stimulated by electricity in the appropriate chemical environment and is the science behind the Meso Scale Discovery platform.

 

We at KCAS perform a wide variety of luminescence testing for single analytes, multiplexed analytes, enzyme activity assays, and cell based assays for support of non-clinical and clinical PK, PD/Biomarker and Immunogenicity testing. We have the experience and expertise to develop de novo methods or help optimize methods to meet the regulatory guidelines.