Colorimetric analysis is a method of determining the concentration of a chemical element or chemical compound in a solution with the aid of a colorizing reagent. It is applicable to both organic compounds and inorganic compounds and may be used with or without an enzymatic step.
At KCAS we perform colorimetric testing for support of tissue analysis, enzymatic activity assays, and quantification of metals. For support of tissue analysis we use a qualified bicinchoninic acid assay (BCA assay) for quantification of total protein in homogenized tissues. A number of our PD biomarker enzyme activity assays use a colorimetric readout. These assays can be used to screen multiple matrix including serum/plasma, whole blood cell lysates, CSF and tissues.
Although colorimetric testing has been performed since the 40’s, this technology still has application today. KCAS has the experience and expertise to increase the sensitivity of colorimetric kits, such as those designed for copper detection. Additionally, we have helped our clients by optimizing in-house legacy methods or academic methods to meet regulatory requirements and/or increasing the range of quantification and robustness of the method.