Cell based assays are highly specialized in vitro methods that can evaluate the functional and biochemical effects of a drug. Biopharmaceuticals LBA Cell Culture Services allow for the complex study of cellular mechanisms, both under physiological and experimental conditions, without the need for laboratory animals.
We at KCAS are experienced using primary cells, immortalized cell lines, transfected cell lines, and live cells (whole blood or isolated Peripheral Blood Mononuclear Cells) for development and qualification or validation of cell-based assays. Cell based assays are frequently used to support Nab (neutralizing antibody) assays, mechanism of action (MOA) studies, relative potency assays, and receptor binding assays.
Our scientific staff has an average of 16+ years of cell based experience including receptor occupancy, cell proliferation, apoptosis, necrosis, exosomes, signal transduction, enzyme activity, antibody-dependent cellular cytotoxicity (ADCC), complement-dependent cytoxicity (CDC), surface phenotyping, intracellular cytokine staining, and in vitro stimulations, among others. The platforms we use for support of cell based assays are flow cytometry, ChemiDoc imager, Nexcelom cell counter, MSD, ELISA, Luminex, and SMCxPRO.