Affinity Capture Elution (ACE) – Bridge Assay

Affinity Capture Elution (ACE) – Bridge Assay

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Affinity capture elution (ACE) is a method used in detecting ADA to increase the assay drug tolerance. The method uses acid disassociation of ADA-therapeutic interaction to allow for preferential capture of ADA by an immobilized version of the dosed therapeutic. A second disassociation step used to enable selective transfer of treated sample to an MSD bridging assay. Following the ACE procedure this assay utilizes biotinylated  and ruthenium labeled drug to detect antidrug antibodies.

KCAS has experience in a variety of different sample preparation techniques to improve detection of ADA in the presence of therapeutic. Our scientists are skilled at optimizing the acid/neutralization steps to assure low affinity binding antibodies are able to be captured and detected in unknown samples. Deep experience in developing drug tolerant methods using both bead based and plate based assay formats.

 

KCAS has successfully developed and validated many ADA methods with sufficient drug tolerance to identify patients with both transient and persistent immune responses. We have a proven track record on developing acid disassociation methods to detect anti-drug antibodies earlier than the terminal phase of a pharmacokinetic profile.