Small Molecule Bioanalysis for Drug Development

More than 45 Years –
From Discovery to Clinical

KCAS Bio offers a full range of small molecule bioanalysis from Discovery to Clinical in compliance with non-GLP and GLP regulations as needed.  We specialize in comprehensive method development to deliver robust assays for qualification, validation, and sample analysis.

Our expertise in analytical approaches for small molecules includes:

  • Hydrophilic Interaction Chromatography (HILIC)
  • Solid phase extraction (SPE), Liquid-Liquid Extraction (LLE), Supported Liquid Extraction (SLE)
  • Derivatizations
  • Fast PK / Screening with the focus more on the profile trends
  • PK/ TK / Extended Studies with focus on actual parameter absolute values
  • Protein Binding (Rapid Equilibrium Dialysis or RED, and BioSPME)
  • Size Exclusion Chromatography (SEC)
  • Matrices – all fluids and all tissue types
  • Micro Sampling (Volumetric absorptive microsampling or VAMS)
  • HRMS

LC-MS/MS
LC-MS/MS is widely used for bioanalysis of small molecules, including pharmacokinetics and pharmacodynamics, in drug discovery, preclinical, and clinical studies. This technology is essential for analyzing drugs and metabolites, biomarkers, and active endogenous compounds in biological fluids and tissues. We employ a range of sample preparation techniques, such as protein precipitation (PPT), solid-phase extraction (SPE), and liquid-liquid extraction (LLE), tailored to meet the specific needs of each study.

LBA
Ligand Binding Assays (LBA) can also support small molecule bioanalysis, though they are more commonly used for biomarker analysis than for pharmacokinetics, primarily due to the limited availability of commercial reagents. For example, LBAs are often employed to quantify eicosanoids or leukotrienes.

With expertise in LC-MS/MS, Ligand Binding Assays, and various sample preparation techniques, we provide accurate and reliable results for pharmacokinetics, pharmacodynamics, and biomarker analysis.

Fill out the form today to inquire about tailored solutions that support your drug development and help drive the success of your research.