Pioneering in Hybrid Technology for 25 Years

Leading the Way in Hybrid LC-MS/MS: Speed, Sensitivity and Flexibility 

KCAS Bio is a leader in Hybrid LC-MS/MS, also known as immunoaffinity or affinity capture LC-MS. This technique enables highly selective and sensitive quantitation of low-level analytes, such as proteins and peptides, in biological matrices. By combining antibody-based enrichment with the precision of LC-MS/MS, it integrates key elements of ligand binding assays (LBA) and mass spectrometry, making it a powerful tool for bioanalysis

KCAS Bio is an industry leading expert in Hybrid LC-MS/MS and has supported many modalities from non-GLP pre-clinical studies to GLP and clinical trials.  At KCAS Bio, we partner with our clients to understand the specific project drivers and their drug development requirements. We evaluate each project by starting with the assessment of feasibility in LC-MS/MS, LBA, or both.

 When is Hybrid LC-MS/MS the best choice for Large Molecule Bioanalysis? 

    • Lack of availability of reagents does not prevent development of an LC-MS/MS assay
    • Feasible to develop LC-MS/MS assay with one or no good reagents
    • Method feasibility/development can start with short lead times
    • Use selectivity from extraction, chromatography and MS/MS to avoid interferences
    • Ready translation between species with some sequence changes. Less concern of effect on Ab binding
    • Ability to differentiate between endogenous (replacement therapy) or human drug in pre-clinical background
    • Ability to work around and detect simple sequence changes (e.g. – 1 amino acid, phosphorylation)
    • Flexibility in analysis – not tied to a single molecular entity – dial in selectivity by picking different surrogate peptides
    • Ability to provide multiple data assessments at any time – Multiplexing
    • Same protein but different parts of the molecule or different proteins

Ready to enhance your drug development with precise bioanalysis? Fill out the form and a scientist will help you explore how our Hybrid LC-MS/MS solutions can accelerate your research and deliver reliable results.