Cell Based Neutralizing Antibodies (Nabs)

Cell Based Neutralizing Antibodies (Nabs)

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Cell based neutralization assays encompass a variety of methods that can be utilized in the development of a drug and can include both neutralizing antibody methods or confirmation of the mechanism of action of a drug. Neutralizing antibody assays utilize a cell based system to identify when a surface receptor is being activated and blocked by known neutralizing antibody. Mechanism of action confirmation and receptor occupancy methods look at a similar cell based system to identify when the target pathway is being activated and confirm the pathway is being induced by a drug through specific inhibition of a therapeutic or receptor.

KCAS scientists have a wealth of experience in developing analytical methods utilizing cells as an important reagent to explain the system biology. Our team of cell culture specialists, have cultured primary cells as well as immortal cell lines for use in imaging and flow cytometry. We know how to build and manage a cell bank to ensure that a? proper passage number of cells are optimized for the cell based method. We can transfer a method and if needed optimize the method to meet regulatory requirements.

 

KCAS has a dedicated, isolated cell culture suite to dramatically reduce the change in potential contamination of the cell during culture and processing. We have multiple tools to assist us in choosing the most appropriate output for your assay, whether that be flow cytometry, imaging, cAMP readouts, etc.