Valproic Acid in Human Plasma Assay

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: K2EDTA

Storage Temperature: -20 °C or -70 °C

LLOQ: 0.500 mcg/mL

ULOQ: 150 mcg/mL

Sample Volume: 100 mcL

Precision & Accuracy:

  • LLOQ QC: 4.8% CV; -5.0% Bias
  • Low QC: 4.7% CV; -3.6% Bias
  • Mid QC: 2.6% CV; 1.4% Bias
  • High QC: 1.8% CV; -0.2% Bias

Stability:

  • Short-Term (Bench-Top): 24 hours
  • Long-Term: 335 days at -20 °C and -70 °C
  • Freeze/Thaw Cycles: 4 Cycles
  • Whole Blood: 1.5 hr at ambient

Comments: Evaluated in the presence of Ethosuximide, Gabapentin, Lamotrigine, Levatiracetam, Oxcarbazepine, Phenytoin, Tiagabine and Topiramate.

Method Number: V2425P1