Tretinoin in Human Plasma Assay

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Sodium Heparin

Storage Temperature: -20 °C

LLOQ: 10 ng/mL

ULOQ: 1000 ng/mL

Sample Volume: 500 mcL

Precision & Accuracy:

  • LLOQ QC: 12.7% CV; 0.9% Bias
  • Low QC: 7.6% CV; -0.4% Bias
  • Mid QC: 6.7% CV; -0.1% Bias
  • High QC: 5.8% CV; 0.7% Bias
  • Above Range QC (10x Dilution): 9.3% CV; 5.8% Bias

Stability:

  • Short-Term (Bench-Top): 5.5 hours
  • Long-Term: 67 days at -20 °C
  • Freeze/Thaw Cycles: 3
  • Comments: Stabilized with Ascorbic Acid

Method Number: V1579P1