Tramadol in Human Plasma Assay

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Potassium EDTA

Storage Temperature: -20 °C or -70 °C

LLOQ: 2.5 ng/mL

ULOQ: 480 ng/mL

Sample Volume: 100 mcL

Precision & Accuracy:

  • LLOQ QC: 11.6% CV; 3.5% Bias
  • Low QC: 7.6% CV; 0.1% Bias
  • Mid QC: 3.5% CV; -2.1% Bias
  • High QC: 3.2% CV; -0.7% Bias
  • Above Range QC (5x Dilution): 3.4% CV; -5.3% Bias

Stability:

  • Short-Term (Bench-Top): 27 hours and 30 minutes
  • Long-Term: 136 days at -20 °C; 136 days at -70 °C
  • Freeze/Thaw Cycles: 4

Comments: Assay quantitates Acetaminophen (50.0 – 9600 ng/mL)

Method Number: V2339P1