Total Doxorubicinol in Human Plasma

 

 

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Potassium EDTA

Storage Temperature: -20 °C or -70 °C

LLOQ: 0.500 ng/mL

ULOQ: 256 ng/mL

Sample Volume: 150 mcL

Precision & Accuracy:

  • LLOQ QC: 12.1% CV; -3.9% Bias
  • Low QC: 6.4% CV; 1.3% Bias
  • Mid QC: 2.7% CV; 0.0% Bias
  • High QC: 2.5% CV; -0.9% Bias
  • Above Range QC (5x Dilution): 2.2% CV; -1.8% Bias

Stability:

  • Short-Term (Bench-Top): 7 hours
  • Long-Term: 79 days at -20 °C; 266 days at -70 °C
  • Freeze/Thaw Cycles: 4

Method Number: V2359P1