Total Doxorubicin in Human Plasma Assay

 

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Potassium EDTA

Storage Temperature: -20 °C or -70 °C

LLOQ: 100 ng/mL

ULOQ: 51,200 ng/mL

Sample Volume: 100 mcL

Precision & Accuracy:

  • LLOQ QC: 6.2% CV; 7.1% Bias
  • Low QC: 5.6% CV; -4.9% Bias
  • Mid QC: 5.1% CV; 1.0% Bias
  • High QC: 3.1% CV; 2.6% Bias
  • Above Range QC (5x Dilution): 4.0% CV; -12.0% Bias

Stability:

  • Short-Term (Bench-Top): 4 hours
  • Long-Term: 265 days at -20 °C ; 323 days at -70 °C
  • Freeze/Thaw Cycles: 6

Comments: Validated for Doxil® Liposomal Product

Method Number: V2359P2