Tizanidine in Human Plasma

 

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Sodium Heparin

Storage Temperature: -20 °C

LLOQ: 0.1 ng/mL

ULOQ: 10 ng/mL

Sample Volume: 500 mcL

Precision & Accuracy:

  • LLOQ QC: 9.1% CV; 10.0% Bias
  • Low QC: 7.1% CV; -3.4% Bias
  • Mid QC: 4.4% CV; -4.8% Bias
  • High QC: 4.1% CV; -11.1% Bias
  • Above Range QC (5x Dilution): 4.4% CV; -14.5% Bias

Stability:

  • Short-Term (Bench-Top): 4.88 hours
  • Long-Term: 4 months at -20 °C
  • Freeze/Thaw Cycles: 3

Comments: Also validated for pediatric studies using 100 mcL sample volumes (analytical range: 0.25 to 10 ng/mL)

Method Number: V1762P1