Tenofovir in Human Plasma Assay

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: K2EDTA

Storage Temperature: -20 °C and -70 °C

LLOQ: 10 ng/mL

ULOQ: 800 ng/mL

Sample Volume: 100 mcL

Precision & Accuracy:

  • LLOQ QC: 4.9% CV; -2.6% Bias
  • Low QC: 4.9% CV; -3.4% Bias
  • Mid QC: 3.2% CV; -3.4% Bias
  • High QC: 2.1% CV; -1.6% Bias
  • Above Range QC (5x Dilution): 4.9% CV; 0.5% Bias

Stability:

  • Short-Term (Bench-Top): 16.25 hours
  • Long-Term: 46 days at -20 °C and -70 °C
  • Freeze/Thaw Cycles: 4

Method Number: V2375P1