Temazepam in Human Plasma

 

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Potassium EDTA

Storage Temperature: -20 °C or -70 °C

LLOQ: 20.0 ng/mL

ULOQ: 1280 ng/mL

Sample Volume: 100 mcL

Precision & Accuracy:

  • LLOQ QC: 6.5% CV; -7.5% Bias
  • Low QC: 4.7% CV; -2.4% Bias
  • Mid QC: 4.0% CV; 3.1% Bias
  • High QC: 2.4% CV; 0.4% Bias
  • Above Range QC (5x Dilution): 2.5% CV; -1.1% Bias

Stability:

  • Short-Term (Bench-Top): 28 hours and 15 minutes
  • Long-Term: 35 days at -20 ºC; 35 days at -70 °C
  • Freeze/Thaw Cycles: 4

Method Number: V2346P1