Tacrolimus in Human Whole Blood Assay

 

 

 

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Whole Blood

Anticoagulant: Potassium EDTA

Storage Temperature: -20 °C or -70 °C

LLOQ: 0.500 ng/mL

ULOQ: 48.0 ng/mL

Sample Volume: 150 mcL

Precision & Accuracy:

  • LLOQ QC: 5.1% CV; -1.7% Bias
  • Low QC: 4.6% CV; 2.5% Bias
  • Mid QC: 3.8% CV; 3.2% Bias
  • High QC: 4.3% CV; -2.0% Bias
  • Above Range QC (5x Dilution): 1.2% CV; 2.8% Bias

Stability:

  • Short-Term (Bench-Top): 26 hours and 42 minutes
  • Long-Term: 488 days at -20 °C and -70 °C
  • Freeze/Thaw Cycles: 4

Comments: Also validated in dog whole blood

Method Number: V2341B1