Tacrolimus in Dog Whole Blood Assay

 

 

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Dog Whole Blood

Anticoagulant: Potassium EDTA

Storage Temperature: -70 °C

LLOQ: 0.100 ng/mL

ULOQ: 19.2 ng/mL

Sample Volume: 150 mcL

Precision & Accuracy:

  • LLOQ QC: 6.0% CV; -3.0% Bias
  • Low QC: 4.1% CV; 1.6% Bias
  • Mid QC: 4.6% CV; 0.9% Bias
  • High QC: 3.7% CV; -5.0% Bias
  • Above Range QC (5x Dilution): 5.9% CV; -13.3% Bias

Stability:

  • Short-Term (Bench-Top): 23 hours
  • Long-Term: 133 days at -70 °C
  • Freeze/Thaw Cycles: 4

Comments: Also validated in human whole blood

Method Number: V2341B1