Sumatriptan in Human Plasma Assay

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Potassium EDTA

Storage Temperature: -20 °C or -70 °C

LLOQ: 0.500 ng/mL

ULOQ: 96.0 ng/mL

Sample Volume: 200 mcL

Precision & Accuracy:

  • LLOQ QC: 5.7% CV; 1.3% Bias
  • Low QC: 4.1% CV; -3.0% Bias
  • Mid QC: 2.2% CV; -0.4% Bias
  • High QC: 3.2% CV; -1.3% Bias
  • Above Range QC (5x Dilution): 2.2% CV; 2.7% Bias

Stability:

  • Short-Term (Bench-Top): 28 hours
  • Long-Term: 128 days at -20 °C; 128 days at -70 °C
  • Freeze/Thaw Cycles: 4

Method Number: V2348P1