Spironolactone in Human Plasma Assay

 

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: K2EDTA

Storage Temperature: -20 C and -70 °C

LLOQ: 0.5 ng/mL

ULOQ: 128 ng/mL

Sample Volume: 200 mcL

Precision & Accuracy:

  • LLOQ QC: 8.5% CV; 2.7% Bias
  • Low QC: 4.8% CV; -3.7% Bias
  • Mid QC: 3.3% CV; 3.3% Bias
  • High QC: 6.1% CV; 2.0% Bias
  • Above Range QC (5x Dilution): 2.1% CV; -5.5% Bias

Stability:

  • Short-Term (Bench-Top): 24 hours
  • Long-Term: 25 days at -20 °C and -70 °C
  • Freeze/Thaw Cycles: 4

Method Number: V2349P1