Simvastatin in Human Plasma Assay

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Potassium K2EDTA

Storage Temperature: -70 °C

LLOQ: 50 pg/mL

ULOQ: 25,600 pg/mL

Sample Volume: 500 mcL

Precision & Accuracy:

  • LLOQ QC: 8.9% CV; -3.4% Bias
  • Low QC: 6.7% CV; 2.3% Bias
  • Mid QC: 4.4% CV; 2.5% Bias
  • High QC: 4.3% CV; -1.3% Bias
  • Above Range QC (5x Dilution): 2.0% CV; 6.3% Bias

Stability:

  • Short-Term (Bench-Top): 7 hours (RT); 24 hour (Ice Bath)
  • Long-Term: at -70 °C
  • Freeze/Thaw Cycles: 4 (-70 °C / Ice Bath)

Method Number: V2352P2