Ropinirole in Human Plasma Assay

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Potassium EDTA

Storage Temperature: -20 °C or -70 °C

LLOQ: 20 pg/mL

ULOQ: 5120 pg/mL

Sample Volume: 200 mcL

Precision & Accuracy:

  • LLOQ QC: 8.4% CV; 7.3% Bias
  • Low QC: 6.5% CV; 1.9% Bias
  • Mid QC: 7.5% CV; -1.6% Bias
  • High QC: 5.1% CV; -1.7% Bias
  • Above Range QC (5x Dilution): 6.3% CV; -5.9% Bias

Stability:

  • Short-Term (Bench-Top): 22 hours and 35 minutes
  • Long-Term: 316 days at -20 °C; 316 days at -70 °C
  • Freeze/Thaw Cycles: 4

Method Number: V2293P1