Ribavirin in Human Plasma Assay

 

 

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: K2EDTA or K3EDTA

Storage Temperature: -20 °C or -70 °C

LLOQ: 5 ng/mL

ULOQ: 2240 ng/mL

Sample Volume: 150 mcL

Precision & Accuracy:

  • LLOQ QC: 6.3% CV; 1.9% Bias
  • Low QC: 4.2% CV; 0.0% Bias
  • Mid QC: 2.0% CV; 4.2% Bias
  • High QC: 3.3% CV; 0.4% Bias
  • Above Range QC (5x Dilution): 2.4% CV; -4.3% Bias

Stability:

  • Short-Term (Bench-Top): 21.75 hours
  • Long-Term: 95 days at -20 °C and -70 °C
  • Freeze/Thaw Cycles: 6

Comments: Investigator assays also developed for mouse plasma, mouse lung and cell media

Method Number: V2347P1