Pseudoephedrine in Human Plasma Assay

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Sodium Heparin

Storage Temperature: -20 °C or -70 °C

LLOQ: 5.00 ng/mL

ULOQ: 500 ng/mL

Sample Volume: 200 mcL

Precision & Accuracy:

  • LLOQ QC: 6.2% CV; 4.5% Bias
  • Low QC: 5.2% CV; 0.1% Bias
  • Mid QC: 3.2% CV; -1.1% Bias
  • High QC: 2.7% CV; 0.0% Bias
  • Above Range QC (5x Dilution): 3.0% CV; 1.3% Bias

Stability:

  • Short-Term (Bench-Top): 5 hours and 30 minutes
  • Long-Term: 327 days at -20 °C; 235 days at -70 °C
  • Freeze/Thaw Cycles: 3

Comments: Assay also quantitates Chlorpheniramine and Brompheniramine (0.5 to 50 ng/mL, each).

Method Number: V2212P1