Propoxyphene in Human Plasma Assay

 

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Sodium Heparin

Storage Temperature: -20 °C

LLOQ: 1 ng/mL

ULOQ: 250 ng/mL

Sample Volume: 200 mcL

Precision & Accuracy:

  • LLOQ QC: 14.1% CV; -1.0% Bias
  • Low QC: 7.0% CV; -2.8% Bias
  • Mid QC: 3.5% CV; 0.0% Bias
  • High QC: 1.9% CV; -0.2% Bias
  • Above Range QC (5x Dilution): 5.4% CV; 6.4% Bias

Stability:

  • Short-Term (Bench-Top): 4 hours
  • Long-Term: 6 months at -20 °C
  • Freeze/Thaw Cycles: 4

Comments: Validated for a high curve (10 to 2500 ng/mL), in presence of Acetaminophen and using Li Heparin Anticoagulant

Method Number: V2136P1