Phenylephrine in Human Urine Assay

 

 

 

 

Method: LC-MS/MS

Matrix: Human Urine

Storage Temperature: -20 °C

LLOQ: 5 ng/mL

ULOQ: 1000 ng/mL

Sample Volume: 250 mcL

Precision & Accuracy:

  • LLOQ QC: 13.7% CV; -3.6% Bias
  • Low QC: 6.5% CV; -1.8% Bias
  • Mid QC: 5.8% CV; -1.1% Bias
  • High QC: 5.8% CV; 0.5% Bias
  • Above Range QC (5x Dilution): 11.6% CV; 7.7% Bias

Stability:

  • Short-Term (Bench-Top): 4.5 hours
  • Long-Term: 147 days at -20 °C
  • Freeze/Thaw Cycles: 3

Comments: Validated in the presence of Aspirin, Salicylic Acid and Chlorpheniramine

Method Number: V1870U1