Oxypurinol in Human Plasma Assay

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Sodium Heparin

Storage Temperature: -20 °C

LLOQ: 0.1 mcg/mL

ULOQ: 10 mcg/mL

Sample Volume: 100 mcL

Precision & Accuracy:

  • LLOQ QC: 12.7% CV; 1.1% Bias
  • Low QC: 8.3% CV; 5.4% Bias
  • Mid QC: 5.4% CV; 2.3% Bias
  • High QC: 4.4% CV; -1.7% Bias
  • Above Range QC (10x Dilution): 3.1% CV; 5.8% Bias

Stability:

  • Short-Term (Bench-Top): 25 hours and 20 minutes
  • Long-Term: 315 days at -20 °C
  • Freeze/Thaw Cycles: 3

Comments: Assay quantitates Allopurinol (0.05 to 5 mcg/mL). Also validated in Li Heparin Plasma.

Method Number: V2205P1