Oxycodone in Human Plasma Assay

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Sodium Heparin

Storage Temperature: -20 °C

LLOQ: 0.250 ng/mL

ULOQ: 50.0 ng/mL

Sample Volume: 500 mcL

Precision & Accuracy:

  • LLOQ QC: 12.5% CV; -4.0% Bias
  • Low QC: 8.5% CV; -6.0% Bias
  • Mid QC: 3.5% CV; -1.3% Bias
  • High QC: 4.9% CV; -1.2% Bias

Stability:

  • Short-Term (Bench-Top): 4 hours and 15 minutes
  • Long-Term: 20 weeks at -20 °C
  • Freeze/Thaw Cycles: 3

Comments: Assay quantitates Noroxycodone and Oxymorphone metabolites (0.250 – 50 ng/mL each). Assay also validated in the presence of Naltrexone and 6-β-Naltrexol.

Method Number: V2043P1