Oxybutynin in Human Plasma Assay

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Potassium EDTA

Storage Temperature: -70 °C

LLOQ: 50 pg/mL

ULOQ: 6400 pg/mL

Sample Volume: 400 mcL

Precision & Accuracy:

  • LLOQ QC: 12.8% CV; -5.0% Bias
  • Low QC: 7.3% CV; 1.3% Bias
  • Mid QC: 6.4% CV; 2.9% Bias
  • High QC: 6.3% CV; 1.6% Bias
  • Above Range QC (5x Dilution): 4.4% CV; -1.8% Bias

Stability:

  • Short-Term (Bench-Top): 22 hours and 50 minutes
  • Long-Term: 51 days at -70 °C
  • Freeze/Thaw Cycles: 5

Comments: Assay quantitates Desethyloxybutynin (50 – 6400 pg/mL).

Method Number: V2306P2