Omeprazole in Human Plasma Assay

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Sodium Heparin (and Lithium Heparin)

Storage Temperature: -20 °C

LLOQ: 5 ng/mL

ULOQ: 500 ng/mL

Sample Volume: 500 mcL

Precision & Accuracy:

  • LLOQ QC: 9.9% CV; 4.8% Bias
  • Low QC: 5.6% CV; -1.1% Bias
  • Mid QC: 6.8% CV; 1.1% Bias
  • High QC: 6.6% CV; -0.5% Bias
  • Above Range QC (5x Dilution): 3.6% CV; 4.6% Bias

Stability:

  • Short-Term (Bench-Top): 4 hours
  • Long-Term: 2 months at -20 °C
  • Freeze/Thaw Cycles: 3

Comments: Also validated for Hydroxyomeprazole, Omperazole Sulfone, Omeprazole Sulfide and 5-O-Desmethylomeprazole.

Method Number: V1652P1