Norpropoxyphene in Human Plasma Assay

 

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Sodium Heparin

Storage Temperature: -20 °C

LLOQ: 1 ng/mL

ULOQ: 250 ng/mL

Sample Volume: 200 mcL

Precision & Accuracy:

  • LLOQ QC: 11.0% CV; 9.0% Bias
  • Low QC: 7.5% CV; 1.2% Bias
  • Mid QC: 4.5% CV; 1.2% Bias
  • High QC: 2.8% CV; 3.2% Bias
  • Above Range QC (5x Dilution): 6.5% CV; 10.3% Bias

Stability:

  • Short-Term (Bench-Top): 4 hours
  • Long-Term: 6 months at -20 °C
  • Freeze/Thaw Cycles: 4

Comments: Validated for a high curve (10 to 2500 ng/mL), in presence of Acetaminophen and using Li Heparin Anticoagulant

Method Number: V2136P1