Naproxen in Human Plasma Assay

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Sodium Heparin

Storage Temperature: -20 °C or -70 °C

LLOQ: 250 ng/mL

ULOQ: 64,000 ng/mL

Sample Volume: 150 mcL

Precision & Accuracy:

  • LLOQ QC: 5.3% CV; 5.7% Bias
  • Low QC: 3.5% CV; -1.5% Bias
  • Mid QC: 2.1% CV; -0.4% Bias
  • High QC: 2.0% CV; -0.3% Bias
  • Above Range QC (5x Dilution): 2.3% CV; -9.3% Bias

Stability:

  • Short-Term (Bench-Top): 25 hours
  • Long-Term: 95 days at -20 °C and -70 °C
  • Freeze/Thaw Cycles: 5

Comments: Validated in presence of Guaifenesin

Method Number: V2361P2