Diltiazem, N-Desmethyl Human Plasma Assay Performance

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Potassium EDTA

Storage Temperature: -20°C

LLOQ: 2.00 ng/mL

ULOQ: 256 ng/mL

Sample Volume: 200 mcL

Precision & Accuracy:

  • LLOQ QC: 12.4% CV; 0.1% Bias
  • Low QC: 5.6% CV; 1.4% Bias
  • Mid QC: 6.0% CV; 3.6% Bias
  • High QC: 5.8% CV; 7.4% Bias
  • Above Range QC (5x Dilution): 10.6% CV; 7.4% Bias

Stability:

  • Short-Term (Ice Bath): 6 hours and 45 minutes
  • Long-Term: 59 days at -20°C
  • Freeze/Thaw Cycles: 4

Comments: Assay quantitates Diltiazem (4.00-512 ng/mL) and Desacetyl Diltiazem (1.00-128 ng/mL).

Method Number: V2330P3