Morphine in Human Plasma Assay

 

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Potassium EDTA

Storage Temperature: -70 °C

LLOQ: 0.5 ng/mL

ULOQ: 96 ng/mL

Sample Volume: 250 mcL

Precision & Accuracy:

  • LLOQ QC: 11.2% CV; 0.8% Bias
  • Low QC: 8.7% CV; -0.5% Bias
  • Mid QC: 3.2% CV; -0.8% Bias
  • High QC: 3.3% CV; -2.2% Bias
  • Above Range QC (5x Dilution): 3.4% CV; 3.1% Bias

Stability:

  • Short-Term (Bench-Top): 25 hours and 15 minutes
  • Long-Term: 622 days at -70 °C
  • Freeze/Thaw Cycles: 4

Method Number: V2323P1