Morphine 3G in Human Plasma Assay

 

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Potassium EDTA

Storage Temperature: -70 °C

LLOQ: 5 ng/mL

ULOQ: 960 ng/mL

Sample Volume: 250 mcL

Precision & Accuracy:

  • LLOQ QC: 6.5% CV; -2.8% Bias
  • Low QC: 4.4% CV; 0.6% Bias
  • Mid QC: 3.4% CV; -0.4% Bias
  • High QC: 3.2% CV; -5.8% Bias
  • Above Range QC (5x Dilution): 2.0% CV; 1.9% Bias

Stability:

  • Short-Term (Bench-Top): 25 hours and 15 minutes
  • Long-Term: Under Investigation at -70 °C
  • Freeze/Thaw Cycles: 4

Method Number: V2323P1