Midazolam in Human Plasma

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Sodium Heparin

Storage Temperature: -20 °C

LLOQ: 0.5 ng/mL

ULOQ: 500 ng/mL

Sample Volume: 500 mcL

Precision & Accuracy:

  • LLOQ QC: 9.4% CV; 6.0% Bias
  • Low QC: 8.3% CV; -4.0% Bias
  • Mid QC: 4.4% CV; -1.7% Bias
  • High QC: 7.0% CV; -5.4% Bias
  • Above Range QC (2x Dilution): 6.6% CV; -6.9% Bias

Stability:

  • Short-Term (Bench-Top): 4 hours
  • Long-Term: 13 months at -20 °C
  • Freeze/Thaw Cycles: 3

Comments: Validated for α-Hydroxymidazolam and in presence of Carbamazepine, Lamotrigine, Levetiracetam, Phenytoin, Topiramate and Valproic Acid

Method Number: V1573P2