Metoprolol in Human Plasma Assay

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: K2EDTA

Storage Temperature: -20 °C

LLOQ: 1 ng/mL

ULOQ: 320 ng/mL

Sample Volume: 100 mcL

Precision & Accuracy:

  • LLOQ QC: 11.4% CV; -1.7% Bias
  • Low QC: 7.1% CV; 0.0% Bias
  • Mid QC: 5.6% CV; 4.3% Bias
  • High QC: 6.5% CV; -2.2% Bias
  • Above Range QC (5x Dilution): 9.8% CV; -1.2% Bias

Stability:

  • Short-Term (Bench-Top): 23 hours and 30 minutes
  • Long-Term: 97 days at -20 °C
  • Freeze/Thaw Cycles: 3

Method Number: V2289P1