Metformin in Human Plasma Assay

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Potassium EDTA

Storage Temperature: -20 °C

LLOQ: 20 ng/mL

ULOQ: 5,120 ng/mL

Sample Volume: 150 mcL

Precision & Accuracy:

  • LLOQ QC: 8.9% CV; -5.5% Bias
  • Low QC: 6.7% CV; -2.1% Bias
  • Mid QC: 5.2% CV; -0.6% Bias
  • High QC: 4.2% CV; 0.0% Bias
  • Above Range QC (5x Dilution): 4.4% CV; -11.1% Bias

Stability:

  • Short-Term (Bench-Top): 6 hours
  • Long-Term: 105 days at -20 °C
  • Freeze/Thaw Cycles: 4

Method Number: V2367P1