Losartan in Human Plasma Assay

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: K2EDTA

Storage Temperature: -20 °C and -70 °C

LLOQ: 2 ng/mL

ULOQ: 750 ng/mL

Sample Volume: 100 mcL

Precision & Accuracy:

  • LLOQ QC: 9.0% CV; 2.9% Bias
  • Low QC: 4.4% CV; -1.0% Bias
  • Mid QC: 4.1% CV; 0.3% Bias
  • High QC: 3.5% CV; -1.2% Bias
  • Above Range QC (5x Dilution): 3.8% CV; 4.6% Bias

Stability:

  • Short-Term (Bench-Top): 6 hours
  • Long-Term: 39 days at -20 °C and -70 °C
  • Freeze/Thaw Cycles: 5

Comments: Validated for the quantitation of Losartan Carboxylic Acid and in the presence of Atorvastatin and its 2- and 4-Hydroxy metabolites

Method Number: V2398P1