Losartan Carboxylic Acid in Human Plasma Assay

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: K2EDTA

Storage Temperature: -20 °C and -70 °C

LLOQ: 2 ng/mL

ULOQ: 750 ng/mL

Sample Volume: 100 mcL

Precision & Accuracy:

  • LLOQ QC: 9.1% CV; 4.8% Bias
  • Low QC: 4.8% CV; -0.1% Bias
  • Mid QC: 3.9% CV; 1.9% Bias
  • High QC: 2.5% CV; -1.9% Bias
  • Above Range QC (5x Dilution): 1.2% CV; 6.3% Bias

Stability:

  • Short-Term (Bench-Top): 6 hours
  • Long-Term: 39 days at -20 °C and -70 °C
  • Freeze/Thaw Cycles: 5

Comments: Validated for the quantitation of Losartan and in the presence of Atorvastatin and its 2- and 4-Hydroxy metabolites

Method Number: V2398P1