Ketoconazole in Human Plasma Assay

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Potassium EDTA

Storage Temperature: -20 °C or -70 °C

LLOQ: 50 ng/mL

ULOQ: 16,000 ng/mL

Sample Volume: 100 mcL

Precision & Accuracy:

  • LLOQ QC: 10.9% CV; -2.1% Bias
  • Low QC: 5.8% CV; 8.1% Bias
  • Mid QC: 3.9% CV; 9.4% Bias
  • High QC: 3.2% CV; -2.1% Bias
  • Above Range QC (5x Dilution): 2.8% CV; -1.3% Bias

Stability:

  • Short-Term (Bench-Top): 24 hours
  • Long-Term: 23 days at -20 °C or -70 °C
  • Freeze/Thaw Cycles: 4

Comments: Validated in the presence of Clotrimazole

Method Number: V2353P1