Isradipine in Human Plasma Assay

 

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Potassium EDTA

Storage Temperature: -20 °C

LLOQ: 0.10 ng/mL

ULOQ: 20 ng/mL

Sample Volume: 400 mcL

Precision & Accuracy:

  • LLOQ QC: 13.2% CV; -6.5% Bias
  • Low QC: 8.7% CV; 12.5% Bias
  • Mid QC: 6.6% CV; 3.4% Bias
  • High QC: 3.2% CV; 1.1% Bias
  • Above Range QC (8x Dilution): 7.9% CV; -5.1% Bias

Stability:

  • Short-Term (Bench-Top): 5 hours and 15 minutes
  • Long-Term: 124 days at -20 °C
  • Freeze/Thaw Cycles: 3

Comments: Lower range assay (0.050 to 10 ng/mL) also validated using 600 mcL plasma

Method Reference: V2184P1