Indomethacin in Human Plasma Assay

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Potassium EDTA

Storage Temperature: -20 °C and -70 °C

LLOQ: 10 ng/mL

ULOQ: 4480 ng/mL

Sample Volume: 150 mcL

Precision & Accuracy:

  • LLOQ QC: 5.4% CV; -1.0% Bias
  • Low QC: 3.2% CV; -1.8% Bias
  • Mid QC: 2.3% CV; 2.1% Bias
  • High QC: 1.8% CV; 0.2% Bias
  • Above Range QC (5x Dilution): 4.1% CV; -2.4% Bias

Stability:

  • Short-Term (Bench-Top): 27 hours
  • Long-Term: Under investigation at both -20 °C and -70 °C
  • Freeze/Thaw Cycles: 4

Method Number: V2351P1